Denaturation Step, professor Pear: Remember that PCR is kind of like artificial replication in a tube. Professor Pear: If we again use DNA replication as a guide, the next step that needs to occur is that primers need to attach to the DNA template. Look at the PCR products. It's true that we're not adding helicase, but there's a really good reason. Professor Pear: Oh, my pleasure. This is known as the denaturation step in PCR. This step would kill the protein because a human protein isn't designed to function at this temperature. While they're really cool, enzymes are kind of expensive and sometimes finicky to work with. In replication, an enzyme - RNA primase - creates the primer nucleotide-by-nucleotide on the DNA template.
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Shop BY brand, receive 10 OFF, copyright. In this lesson, you will learn about the steps required to amplify DNA during PCR. To make millions of copies, we need to perform these three steps multiple times. That means that we have the two original DNA strands again as templates, but we also have these two new strands that are a little shorter. Reporter: But, we're not adding helicase to the PCR habitat discount code tube, so it's not going to work! The cycling nature of PCR enables the procedure to specifically produce millions of copies of the target DNA.